| Gene name: | SIRT2 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | NP |
| Gene ID: | 22933 |
| Category: | protein coding |
| Phenotype: | Disc degenerative disease |
| Experimental category: | L |
| PMID: | 30981502 |
| Experiment: | SA-β-gal activity assay//Western blot//Knockdown//RT-PCR//Flow cytometry |
| Description: | NP cells expressed significantly higher β-gal and MMP3/9 levels with higher IL-1b stimulation. NP cells were transfected with Lenti-sirt2 to overexpress Sirt2 protein. Meanwhile, Lenti-NC was used as the sham group. The function of lentivirus transfection was verified through WB analysis. Results indicated that these cells exhibited significantly less β-gal, MMP3/9 activity than those in IL-1b-induced group. Furthermore, IF staining, WB and RT-PCR analysis demonstrated that the expression of type II collagen was significantly up-regulated.Flow cytometry result showed that compared with the control group, more cells stayed in the G1 phase and less cells stayed in the M phase in the two IL-1b stimulation groups. Total ROS level increased remarkably under the treatment of IL-1b. |
| Target gene: | IL-1β//SOD1//SOD2 |
| Official symbol(s): | IL1B//SOD1//SOD2 |
| R-AG-Target gene: | Downregulation//Upregulation//Upregulation |
| Subcategory: | Unclear |
| Target gene experiment: | RT-PCR//Western blot//Flow cytometry |
| Target gene description: | Results indicated that these cells exhibited significantly less β-gal, MMP3/9 activity than those in IL-1b-induced group.Sirt2 expression decreased significantly in cells after treatment with IL-1b. Meanwhile, total ROS level decreased obviously under the treatment of Lenti-sirt2.In addition, anti-oxidative SOD1 and SOD2 were remarkably up-regulated compared with IL-1b- induced group. |
| Regulatory pathway: | P53-P21 |
| R-AG-Pathway: | Downregulation |
| Official symbol(s): | TP53-CDKN1A |
| Pathway experiment: | Western blot//RT-PCR |
| Pathway description: | Western blot and RT-PCR results indicated that Sirt6 overexpression significantly decreased the expressions of p53 and p21. |
Annotation:
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