| Gene name: | PRDX6 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | Gastrocnemius |
| Cell name: | C2C12 |
| Gene ID: | 9588 |
| Category: | protein coding |
| Phenotype: | Metabolic Sarcopenia |
| Experimental category: | HL |
| PMID: | 32316601 |
| Experiment: | SA-β-gal activity assay//Knockdown//qRT-PCR |
| Description: | We screened the expression of 84 key genes involved in the senescence program on gastrocnemius of wt and Prdx6-/- mice. A significant up-regulation of p53 (p < 0.05),Cdkn1b (p < 0.05) and Cdkn1c (p < 0.05), was found in Prdx6-/- mice.Prdx6-/- mice showed increase in activity of SA-β-Gal compared to wt mice (p < 0.005).The expression levels of telomeric repeat-binding factor 1 (TERF1), telomeric repeat-binding factor 2-interacting protein 1 (TERF2IP), telomerase-associated protein 1 (TEP1), and regulator of telomere length 1 (RTEL1), were significantly reduced in Prdx6-/-mice compared to wt mice (p < 0.05). |
| Target gene: | SIRT1 |
| Official symbol(s): | SIRT1 |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot//Knockdown |
| Target gene description: | Following the nuclear and cytoplasmic proteins fractionation, indeed, we found that in Prdx6-/- mice SIRT1 localized predominantly in the cytoplasm (p < 0.05) rather than in the nucleus (p < 0.0005) compared to wt, suggesting reduced levels of Prdx6 lead to an impairment of SIRT1 nuclearcytoplasmic shuttling. |
| Regulatory pathway: | P53-P21 |
| R-AG-Pathway: | -- |
| Official symbol(s): | TP53-CDKN1A |
| Pathway experiment: | Western blot//Knockdown |
| Pathway description: | As expected, p53 acetylation was significantly increased in Prdx6-/- mice compared to wt mice, confirming the SIRT1 reduced nuclear activity. Acetylated p53 induces p21, a cyclin-dependent kinase inhibitor 1 able to regulate cell cycle and directly blunt telomerase activity . Consistently with the reduction of ATLR and increase in p53 activation, we observed a significantly enhanced steady-state levels of p21 in Prdx6-/- mice. |
Annotation:
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