| Gene name: | TLR4 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | MLEC |
| Gene ID: | 7099 |
| Category: | protein coding |
| Phenotype: | Emphysema and COPD |
| Experimental category: | L |
| PMID: | 30790400 |
| Experiment: | SA-β-gal activity assay//Knockdown//Flow cytometry |
| Description: | TLR4 mouse lung Ec (MLEC) showed increase in SA‐β‐gal activity, even at low passages, compared to WT MLEC.To further investigate the role of TLR4 in cellular senescence, we examined the cell growth rate and cell cycle analysis between TLR4?/? and WT MLEC. TLR4?/? MLEC had a significantly decreased rate of cell growth compared with WT MLEC. Analysis of cell cycle distribution by flow cytometry showed that TLR4?/? MLEC exhibited increased G1 phase (83.4 ± 1.4% vs. 74.5 ± 3.8%) and decreased G2 phase (10.0 ± 1.5% vs. 16.8 ± 2.4%) compared to WT MLEC. |
| Target gene: | P16//HDAC2 |
| Official symbol(s): | P16//HDAC2 |
| R-AG-Target gene: | Downregulation//-- |
| Subcategory: | Acetylation |
| Target gene experiment: | qRT-PCR//Western blot |
| Target gene description: | In contrast, TLR4 overexpression resulted in a significant decrease in both p16INK4a mRNA and protein expression in hLMVEC.We found that HDAC2 mRNA expression significantly decreased in lungs from TLR4?/? mice compared to WT. We also found that phosphorylated (p)and total‐HDAC2 protein expression were significantly decreased in TLR4?/? MLEC compared to WT.As further confirmation, HDAC2 siRNA sig- nificantly increased p16INK4a. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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