| Gene name: | SARS1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HeLa 1.2.11,BJ |
| Experiment: | SA-β-gal activity assay//Knockdown//FISH |
| Description: | We observed an unexpected increase in SA-β-gal activity and increased levels of cellular senescence signaling molecules, such as P21, P16, and β-galactosidase (β-Gal)32–34 at late cell passages of both cells; these changes were even observed in HeLa 1.2.11 cells, which undergo little replicative senescence, suggesting a role of SerRS in promoting cellular senescence beyond its role in translation.Significant telomere shortening was viewed by reduced FISH signals, further indicating that telomeres were globally shortened when SerRS was overexpressed. |
| Target gene: | POT1 |
| Official symbol(s): | POT1 |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | Pull-down assay//Immunostaining//CHIP//Co-IP |
| Target gene description: | SerRS was partially colocalized with POT1 in the nucleus.The interaction between SerRS and POT1 was further confirmed by their Co-IP from HeLa cells.V5-tagged POT1 was able to be coprecipitated with Flag-tagged SerRS via Flag antibody-mediated isolation; the reverse experiment with a V5 antibody produced a complementary result. In HeLa VST cells, the endogenous SerRS proteins could be coprecipitated with endogenous POT1 by POT1 antibody.The GST pull-down assay clearly showed the direct interaction between SerRS and POT1. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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