| Gene name: | TRPC7 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | Skin |
| Cell name: | -- |
| Experiment: | SA-β-gal activity assay//Knockdown//Western blot |
| Description: | In the absence of UVB, skin phenotype was similar between wild-type and TRPC7+/? or TRPC7?/? mice. In wild-type mice, UVB exposure induced severe desquamation and erythema of the skin. However, in TRPC7+/? and TRPC7?/? mice, UVB exposure induced slight or no damage and less epidermal thickening than in wild-type mice.SA-β-gal staining, used to analyze cell senescence, was also lower in the epidermis of TRPC7+/? and TRPC7?/? mice than in that of wild-type mice after UVB exposure.We found that after UVB irradiation, the knockdown of TRPC7 in keratinocytes significantly decreased the percentage of senescent cells,as determined by SA-β-gal staining,and improved the ratio of keratinocyte survival when compared with control cells. Furthermore, the knockdown of TRPC7 inhibited UVB-induced p53 expression in keratinocytes, which was corroborated by our observation that the UVB-induced activation of the p53 pathway was reduced in TRPC7+/? and TRPC7?/? mice. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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