| Gene name: | ITGB4 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | Mice airway epithelia |
| Cell name: | Mice airway epithelial cell |
| Experiment: | SA-β-gal activity assay//Immunostaining//Knockdown |
| Description: | Our results showed that ITGB4 deficient airway epithelial cells presented typical premature senescence. The rate of positive SA-β-gal staining in airway epithelial cells of ITGB4-/- mice was significantly higher than that in control mice.The cellular proliferative capacity of the airway epithelial cells in ITGB4-/- mice was decreased obviously compared with control mice. |
| Regulatory pathway: | P53-P21 |
| R-AG-Pathway: | -- |
| Official symbol(s): | TP53-CDKN1A |
| Pathway experiment: | Knockdown//qRT-PCR//Western blot |
| Pathway description: | Also, we have previously found increased expression of p53 and p21 in airway epithelia of ITGB4-/- mice. Therefore, we further detect the activation of p53/p21 signaling pathway by qPCR and western blotting in HBE cells. We found that p-p53 (the activated form of p53), p53 and p21 were significantly upregulated in ITGB4-silenced HBE cells. The upregulation of p-p53 was the most pronounced. |
Annotation:
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