| Gene name: | SPRY1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | ASC |
| Experiment: | Western blot//SA-β-gal activity assay//Knockdown//Growth curve assay//BrdU assay//ELISA |
| Description: | SPRY1 KO ASCs showed a proliferation arrest,CRISPR-GFP expressing cells served as negative control. Moreover, the anti-proliferative effect observed in SPRY1 KO ASCs was associated with significantly increased numbers of senescence-associated (SA) β-Galactosidase (β-Gal) positive ASCs and decreased Ki67 abundancy.CCL1 was not detectable by ELISAs, IL-8 and CXCL1/GROα, both are signature cytokines of senescent cells, were significantly elevated in SPRY1 KO ASCs compared to control cells. |
| Target gene: | P53//P21//RB |
| Official symbol(s): | P53//P21//RB |
| R-AG-Target gene: | --//--//-- |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot |
| Target gene description: | SPRY1 KO ASCs showed a considerable upregulation of p53 and its target p21Cip1 associated with Rb activation (hypo-phosphorylation). |
| Regulatory pathway: | RAS-MAPK//NF-κB |
| R-AG-Pathway: | --//-- |
| Official symbol(s): | KRAS-MAPK//NFKB1 |
| Pathway experiment: | Knockdown//Western blot |
| Pathway description: | Sprouty1 protein was depleted by both shRNA #1 and shRNA #2 and silencing of Sprouty1 elevated the abundance of phosphorylated ERK1/2 in PM4-stimulated ASCs compared to shCtrl indicating augmented MAPK activation.C/EBPβ protein was significantly elevated in SPRY1 KO ASCs and nuclear translocation of the NF-κB subunit p65 was significantly increased in these cells indicating an Activate NF-κB pathway. |
Annotation:
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