| Gene name: | SATB1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | Brain |
| Cell name: | -- |
| Gene ID: | 6304 |
| Category: | protein coding |
| Phenotype: | Parkinson's disease |
| Experimental category: | HL |
| PMID: | 31543366 |
| Experiment: | Western blot//SA-β-gal activity assay//Knockdown |
| Description: | Given this, we sought to investigate whether SATB1KO DA neurons present the classical features of cellular senescence. First we observed a dramatic increase in acidic lysosomal (SA-β-gal) activity, the hallmark senescence biomarker.We found upregulation of the majority of SASP factors at 50 days of differentiation in SATB1KO DA neurons versus WT neurons. We confirmed SASP activation by western blotting. In the conditioned medium of SATB1KO neurons, we found IGFBP7, which was absent in the medium of WT neurons. |
| Target gene: | P21 |
| Official symbol(s): | P21 |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot//CHIP//Knockdown//Luciferase reporter assay |
| Target gene description: | Both CDKN1A transcription and p21 protein levels were significantly increased in SATB1KO DA neurons.Using ChIP-seq in DA neurons, we found that SATB1 binds the regulatory region of CDKN1A.To determine whether SATB1 directly represses CDKN1A, we performed a luciferase reporter assay and found that, under conditions of SATB1 knockdown, CDKN1A promoter activity was significantly increased . |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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