| Gene name: | WIF1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | LN-229,LN319 |
| Gene ID: | 11197 |
| Category: | protein coding |
| Phenotype: | Glioblastoma |
| Experimental category: | HL |
| PMID: | 21642372 |
| Experiment: | SA-β-gal activity assay//Cell morphological analysis//Immunostaining//Flow cytometry |
| Description: | We detected an increased percentage of senescence-like cells in WIF1-transfected clones with a positive relationship to WIF1 secretion of the respective clones. Similar differences were observed upon double staining of the cells with SA-β-galactosidase and DAPI, which visualizes nuclear morphology. In the LN319- and LN229-derived WIF1-overexpressing clones, we scored an increased population of both multinucleated and SA-β-galactosidase positive cells. |
| Regulatory pathway: | WNT |
| R-AG-Pathway: | Downregulation |
| Official symbol(s): | Wnt |
| Pathway experiment: | Luciferase reporter assay |
| Pathway description: | Indeed, forced expression of WIF1 inhibited Wnt activity in a dose-dependent manner. |
Annotation:
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