| Gene name: | SOX5 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | Glial cell |
| Experiment: | Cell morphological analysis//SA-β-gal activity assay |
| Description: | We performed SA-β-gal staining of these cells and found that there were an increased number of SA-β-gal-positive cells in Ink4a-/- cells infected with Sox5+PDGFB or Sox5 alone.In Arf-/- cells, there was an increased number of SA-β-gal-stained cells in Sox5-infected cells only. |
| Target gene: | P27//AKT |
| Official symbol(s): | P27//AKT |
| R-AG-Target gene: | Upregulation//Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot |
| Target gene description: | When analyzing p27Kip1protein levels, we found that p27Kip1was clearly upregulated in the Sox5-infected Ink4a-/- cells compared with control and PDGFB-infected cells and that it remained high in the Sox5+PDGFB-infected cells.The total and activated levels of Akt were decreased specifically in the Sox5-stimulated Ink4a-/- cells compared with control and PDGFB cells and remained lower in the Sox5+PDGFB-infected cells compared with PDGFB alone. |
| Regulatory pathway: | PDGFB |
| R-AG-Pathway: | -- |
| Official symbol(s): | PDGFB |
| Pathway experiment: | Immunostaining |
| Pathway description: | To study the proliferation status of Sox5 + PDGFB infected Ink4a-/- and Arf-/- cells, expression of Ki67, a marker of cell proliferation, was analyzed with triple immunofluorescence stainings . In Ink4a-/- cells, HA(PDGFB expression) and V5 (Sox5 expression) double-positive cells were completely negative for Ki-67, whereas in Arf-/- cells, about 20% of the HA + V5 double-positive cells also expressed Ki-67 . |
Annotation:
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