| Gene name: | CDC73 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | 293T,A549,HUVEC |
| Experiment: | SA-β-gal activity assay//Knockdown//EdU assay |
| Description: | Higher percentage of positive SA-β-Gal staining cells was observed in all the three CDC73-KD cells. Moreover, reduced EdU incorporation and cell proliferation were detected upon depletion of CDC73 , indicating decreased expression of CDC73 can reduce DNA synthesis, which is also a well-known molecular phenotype of senescent cells. |
| Regulatory pathway: | P53-P21 |
| R-AG-Pathway: | -- |
| Official symbol(s): | TP53-CDKN1A |
| Pathway experiment: | Western blot//Knockdown |
| Pathway description: | However, both mRNA and protein levels of p53 and p21 were remarkably increased after CDC73 knockdown in 293T cells.Similarly, the protein level of p21 was significantly increased in CDC73-deficient HUVECs and A549 cells . These results support the notion that reduced CDC73 expression may induce senescence through the p53-p21 pathway. |
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