| Gene name: | PAK4 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | MEF |
| Experiment: | SA-β-gal activity assay//Cell morphological analysis |
| Description: | We found that Pak4-infected cells stained positive for SA-β-Gal activity.Interestingly, Pak4-expressing cells showed a large and flat shape. |
| Target gene: | P16//P19ARF//RAF |
| Official symbol(s): | P16//P19ARF//RAF |
| R-AG-Target gene: | Upregulation//Upregulation//Activation |
| Subcategory: | Unclear |
| Target gene experiment: | SA-β-gal activity assay//Western blot |
| Target gene description: | As expected, the control wild-type MEFs were readily arrested after infection with activated Pak4 or RasV12.However,when Pak4 was expressed in primary p19ARF/p16INK4a null MEFs ,the cells continued to grow and did not senesce.In addition，activated Pak4 led to a significant increase in p16INK4a and p19ARFlevels.Furthermore, Pak4 was able to induce phosphorylation of overexpressed and endogenous Raf on serine 338 in vivo, and it directly phosphorylated Raf on this site in vitro. In vitro kinase assays indicated that Pak4 could also stimulate Raf kinase activity. |
| Regulatory pathway: | ERK-MAPK |
| R-AG-Pathway: | Activation |
| Official symbol(s): | MAPK1 |
| Pathway experiment: | Western blot//BrdU assay//SA-β-gal activity assay |
| Pathway description: | We found that ERK activity was strongly stimulated in primary cells stably expressing Pak4 .?Furthermore, treating cells with a chemical inhibitor of the MEK-ERK pathway partially abrogated Pak4-induced premature arrest in primary fibroblasts and led to a decrease of about 50% in the number of SA-β-Gal-positive cells compared to what was seen in the absence of the drug. |
Annotation:
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