Aging Overview

Aging gene

Gene name: SATB1
Aging type: Accelerate
Aging characteristic:
Tissue type: --
Cell name: MEF
Gene ID: 6304
Category: protein coding
Phenotype: Breast cancer
Experimental category: L
PMID: 23686316
Experiment: SA-β-gal activity assay//Cell morphological analysis//Flow cytometry
Description: MEFs expressing SATB1 grew markedly slower than controls,stained positive for β-galactosidase activity and had a flat morphology,indicating a large fraction of SATB1-expressing cells displayed signs of senescence.Consistent with this, we observed a large G1/G0 and a diminished S/G2 population.


Regulatory relationship

Target gene: P16
Official symbol(s): P16
R-AG-Target gene: Upregulation
Subcategory: Unclear
Target gene experiment: SA-β-gal activity assay//Western blot//Flow cytometry
Target gene description: In contrast to wild-type MEFs, SATB1 expression did not cause growth inhibition in p16-/-MEFs. Proliferation was comparable between SATB1- and control virus-infected p16-/- MEFs, and no change in senescence markers or cell cycle distribution was observed.

Regulatory pathway: RB-E2F1
R-AG-Pathway: --
Official symbol(s): RB1-E2F1
Pathway experiment: SA-β-gal activity assay//Co-IP
Pathway description: ATB1-expressing TM MEFs infected with RB virus had a markedly higher fraction of senescent cells compared with TM MEFs without SATB1. Both RB-HA and E2F1-HA were immunoprecipitated by Flag-tagged SATB1.The reciprocal interactions were also detected with HA-tagged E2F1 and RB, showing that SATB1 can interact with RB/E2F1 complexes.


Aging network

Annotation:

The green line represents Upregulation.

The purple line represents Downregulation.

The orange line represents Activation.

The yellow line represents Inhibition.

The gray line represents Unclear.



Pathway view

About risk SNP and eQTL

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