| Gene name: | SATB1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | MEF |
| Gene ID: | 6304 |
| Category: | protein coding |
| Phenotype: | Breast cancer |
| Experimental category: | L |
| PMID: | 23686316 |
| Experiment: | SA-β-gal activity assay//Cell morphological analysis//Flow cytometry |
| Description: | MEFs expressing SATB1 grew markedly slower than controls,stained positive for β-galactosidase activity and had a flat morphology,indicating a large fraction of SATB1-expressing cells displayed signs of senescence.Consistent with this, we observed a large G1/G0 and a diminished S/G2 population. |
| Target gene: | P16 |
| Official symbol(s): | P16 |
| R-AG-Target gene: | Upregulation |
| Subcategory: | Unclear |
| Target gene experiment: | SA-β-gal activity assay//Western blot//Flow cytometry |
| Target gene description: | In contrast to wild-type MEFs, SATB1 expression did not cause growth inhibition in p16-/-MEFs. Proliferation was comparable between SATB1- and control virus-infected p16-/- MEFs, and no change in senescence markers or cell cycle distribution was observed. |
| Regulatory pathway: | RB-E2F1 |
| R-AG-Pathway: | -- |
| Official symbol(s): | RB1-E2F1 |
| Pathway experiment: | SA-β-gal activity assay//Co-IP |
| Pathway description: | ATB1-expressing TM MEFs infected with RB virus had a markedly higher fraction of senescent cells compared with TM MEFs without SATB1. Both RB-HA and E2F1-HA were immunoprecipitated by Flag-tagged SATB1.The reciprocal interactions were also detected with HA-tagged E2F1 and RB, showing that SATB1 can interact with RB/E2F1 complexes. |
Annotation:
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