Gene name: | IL1B |
Aging type: | Accelerate |
Aging characteristic: |
Tissue type: | -- |
Cell name: | HUVEC |
Experiment: | SA-β-gal activity assay//Western blot |
Description: | To address this, primary HUVECs were treated with recombinant human IL-1β for 6 days, the cell senescence was confirmed by SA-β-galactosidase activity.Furthermore, protein expression levels of senescence markers, such as p53 and p21, were also significantly upregulated in a time dependent way after IL-1β treatment with peak values of 2.8 fold and 3.1fold respectively. |
Target gene: | CASPASE-1//NLRP3 |
Official symbol(s): | CASPASE-1//NLRP3 |
R-AG-Target gene: | --//-- |
Subcategory: | Unclear |
Target gene experiment: | Western blot |
Target gene description: | Interestingly, in the presence of z-YVAD-fmk, a caspase-1 inhibitor, mature IL-1 processing was abolished in senescent HUVECs but pro-IL-1 expression remained unchanged .SiNLRP3 or siASC transfection in the cells could significantly decrease their protein expression induced by bleomycin. As expected, IL-1 productions were also strongly attenuated in these silent senescent cells, but there was no significant effect in pro-IL-1 expression. |
Regulatory pathway: | -- |
R-AG-Pathway: | -- |
Pathway experiment: | -- |
Pathway description: | -- |
Annotation:
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