| Gene name: | FBXO5 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | RPE |
| Experiment: | Cell morphological analysis//Knockdown//SA-β-gal activity assay//BrdU assay |
| Description: | Cells lacking Emi1 were large and flattened and contained relatively large nuclei, a cellular morphology remi- niscent of cellular senescence.Approximately 37 to 65% of RPE cells stained positive for SA-β-gal at 4 or 5 days after Emi1 siRNA treatment, a phenotype that was recapitulated using two additional siRNA targeting sequences.We extended this analysis and detected a significant reduction in the prolif- erative capacity of RPE cells treated with human Emi1 siRNA. |
| Target gene: | APC/C//E2F//CYCLIN E1//ATM |
| Official symbol(s): | APC//E2F1//CYCLIN E1//ATM |
| R-AG-Target gene: | --//--//--//-- |
| Subcategory: | Unclear |
| Target gene experiment: | Knockdown//Cell morphological analysis//SA-β-gal activity assay//Western blot |
| Target gene description: | Importantly, senescence induction upon Emi1 depletion required APC/C activity, as it was rescued upon codepletion of Cdh1, and was also observed in HCT116 and HeLa cells.Taken together, Emi1 knockdown results in APC/C activation and a consequent decrease in E2F target protein expression with the exception of an increase in cyclin E1 protein levels.A pronounced decrease in the percentage of senescent cells was measured when cells were grown for three days in the presence of KU-55933 before SA-β-gal staining (25% versus 75% in dimethyl sulfoxide-treated cells).In addition, ATM inhibitor-treated cells displayed a change in morphology from round and flattened (senescent-like) to more elongated and spindle shaped. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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