| Gene name: | PROM1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | CD1331 RPC |
| Gene ID: | 8842 |
| Category: | protein coding |
| Phenotype: | Acute kidney injury |
| Experimental category: | HL |
| PMID: | 29431914 |
| Experiment: | SA-β-gal activity assay |
| Description: | The number of β-galactosidase expressing cells undergoing senescence appeared significantly higher in the CD133-Kd cells compared to their CD1331 control.The evaluation of T/S ratio revealed a significant reduction in telomere length in CD133-Kd cells compared to their CD1331controls,suggesting an implication of CD133 in the prevention of cell senescence. |
| Regulatory pathway: | WNT-β-CATENIN |
| R-AG-Pathway: | Activation |
| Official symbol(s): | Wnt-CTNNB1 |
| Pathway experiment: | qRT-PCR//Western blot//Luciferase reporter assay |
| Pathway description: | Interestingly, CD133-Kd cell lines showed a reduced expression of Wnt4 after cisplatin-induced damage in respect to GFP cells. In addition, CD133-Kd cells showed an impaired response to the pharmacological activation of Wnt signaling pathway using CHIR99021 [31].Luciferase activity in CD1331 RPCs was significantly higher than the one in CD133-Kd cell lines, indicating a reduced β-catenin activation in CD133-Kd cells. Western blot analysis after immunoprecipitation of E-cadherin showed the concomitant presence of CD133 and b-catenin, suggesting that CD133 and E-cadherin may form a complex at the membrane level with β-catenin, thus limiting its cytoplasmic degradation. Indeed, β-catenin levels were significantly lower in CD133-Kd cells in respect to CD1331 RPCs both in basal culture conditions and upon stimulation with b-catenin stabilizer CHIR99021 .Indeed, β-catenin levels were significantly lower in CD133-Kd cells in respect to CD1331 RPCs both in basal culture conditions and upon stimulation with b-catenin stabilizer CHIR99021. |
Annotation:
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