| Gene name: | SENP1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | Skin |
| Cell name: | HFF |
| Experiment: | BrdU assay//SA-β-gal activity assay//Cell morphological analysis//Flow cytometry |
| Description: | In addition, shSenp1 caused substantial inhibition of cell proliferation.Cell cycle analysis showed that the fraction of HFFs in the G1 phase of the cell cycle increased from 53% in mock-infected controls to 76% in cells 5 days after infection with Senp1 shRNA.Cells infected with Senp1 shRNAs also exhibited an enlarged, flattened morphology characteristic of HFFs that have undergone replicative senescence.Greater than 90% of HFFs exposed to any of the three Senp1 shRNAs (#2, 3, and 8) exhibited blue-green cytoplasmic staining indicative of SA-βgal activity, whereas the vast majority of mock-infected HFFs or HFFs expressing the scrambled control shRNA were negative for S-Aβgal activity and exhibited normal morphology.Repression of Senp1 induced a gradual increase in cellular autofluorescence compared to shControlinfected HFFs, reaching approximately sixfold at 10 days after shRNA infection. |
| Regulatory pathway: | P53 |
| R-AG-Pathway: | -- |
| Official symbol(s): | TP53 |
| Pathway experiment: | qRT-PCR |
| Pathway description: | Although there was no significant change in levels of p53 mRNA, repression of Senp1 resulted in enhanced transcriptional activity of p53. |
Annotation:
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