| Gene name: | MYBL2 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HAEC |
| Experiment: | SA-β-gal activity assay//Cell morphological analysis |
| Description: | Importantly, the downregulated B-myb cells showed morphological change including a large-flat shape binucleation and polyploidy after 7 days. B-myb-silenced cells also displayed an increase in the proportion of SA-β-gal-positive cells compared with the control. |
| Regulatory pathway: | ROS-P53-P21 |
| R-AG-Pathway: | -- |
| Official symbol(s): | ROS1-TP53-CDKN1A |
| Pathway experiment: | Western blot//SA-β-gal activity assay//Immunostaining |
| Pathway description: | Interestingly, the protein levels of p53 and p21 were significantly increased in B-myb-silenced cells compared with that of the controls.The upregulation of p- p53, p53 and p21 in B- myb knockdown cells could be abolished in the presence of PFTα. PFTα could also obviously attenuate the upregulation of SA- β- gal activity in B- myb knockdown cells. The production of intracellular ROS was significantly increased in B- myb- silenced cells.Bmyb- silenced cells incubated with NAC could significantly diminish the upregulation of p- p53, p53 and p21. |
Annotation:
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