| Gene name: | BAG3 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | A172 |
| Gene ID: | 9531 |
| Category: | protein coding |
| Phenotype: | Glioblastoma |
| Experimental category: | L |
| PMID: | 25412315 |
| Experiment: | SA-β-gal activity assay//Cell morphological analysis//Cell viability assay//Colony formation assay//Flow cytometry//Knockdown |
| Description: | Of note, cells treated with BIS-specific siRNA (SiBIS) showed typical senescence-related phenotypic changes in a time-dependent manner: large, flattened morphology and gradually increased senescence-associated β-galactosidase (SA-β-Gal) staining: 86.8% of cells were positive for SA-β-Gal staining at 5 days after transfection. The proliferation rate was considerably slower in BIS knockdown cells compared in control cells as determined by relative increase in the cell numbers, 1.7-fold and 5.6-fold at day 5, respectively. The colony-forming ability was also prominently suppressed in SiBIS-treated cells, by 92% compared with control siRNA (SiCON)-treated cells. In addition, cell cycle profile demonstrated that the significant accumulation of cells in the G1 phase of the cell cycle accompanied by decrease in the cell populations in S or G2/M phase in SiBIS-treated cells, showing that the proportion of G1 phase was 84.7% and 59.9% in SiBIS- and SiCON-treated cells, respectively, at day 5. |
| Target gene: | P27 |
| Official symbol(s): | P27 |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot//Knockdown |
| Target gene description: | The p27 levels were also progressively accumulated as increasing concentration of SiBIS. |
| Regulatory pathway: | STAT3-SKP2-P27 |
| R-AG-Pathway: | -- |
| Official symbol(s): | STAT3-SKP2-PSMD9 |
| Pathway experiment: | Western blot//Knockdown |
| Pathway description: | Immunoblottig showed that SKP2 levels were prominently decreased as BIS decreased, to 42% of control cells at day 5, which was inversely correlated with p27.We found that the phosphorylation of STAT3, representing the activated form of STAT3 as a transcriptional regulator, was profoundly decreased by BIS depletion in a time-dependent manner as determined by immunoblotting using specific antibodies for phospho-STAT3 (p-STAT3) that target pS727 and pY705.The depletion of STAT3 activation, accompanied with a decrease in Activate p-STAT3, both pY705 and pS727, downregulated SKP2 but increased p27 expression, comparable to what was observed following BIS depletion. |
Annotation:
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