| Gene name: | BRCA1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | MEC |
| Gene ID: | 672 |
| Category: | protein coding |
| Phenotype: | Breast cancer |
| Experimental category: | L |
| PMID: | 27811360 |
| Experiment: | SA-β-gal activity assay//Cell morphological analysis |
| Description: | We observed that Brca1MGKO MECs exhibited a large and flattened shape, a typical morphology of cellular senescence, while MECs from the other genotypes of mice were smaller and spindle-shaped. Notably, most Brca1MGKO MECs exhibited strong, peri-nuclear staining of SA-β-gal whereas only a small population of WT MECs and very few p16 -/- and p16 -/-;Brca1MGKO MECs showed positive staining. |
| Target gene: | P16 |
| Official symbol(s): | P16 |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | Immunostaining//SA-β-gal activity assay//BrdU assay//Flow cytometry |
| Target gene description: | We found that the percentages of Ki67-positive MECs in p16 -/- and p16 -/- ;Brca1MGKO mice (24.28±2.1 and 24.06±4.3) were significantly higher than those in WT mice (6.39±1.3), which in turn were significantly higher than the percentage in Brca1MGKO (2.20±0.2) mice. Notably, most Brca1MGKO MECs exhibited strong, peri-nuclear staining of SA-β-gal whereas only a small population of WT MECs and very few p16 -/- and p16 -/-;Brca1MGKO MECs showed positive staining. Importantly, MECs from p16 -/- and p16 -/-;Brca1MGKO mice displayed similar BrdU incorporation rates, 81% for p16-/-and 79% for p16-/-;Brca1MGKO, which were significantly higher than their WT counterparts. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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