Gene name: | NFKB2 |
Aging type: | Prevent |
Aging characteristic: |
Tissue type: | -- |
Cell name: | A375 |
Experiment: | Flow cytometry//SA-β-gal activity assay//Western blot//qRT-PCR |
Description: | Cell size and granularity as determined by flow cytometry were significantly increased, as were the senescenceassociated β-galactosidase (SA-β-Gal) activity and HP1γ expression.The mRNA levels of p16 and p21 were increased after NF-kB2 silencing. |
Target gene: | EZH2//NIK |
Official symbol(s): | EZH2//MAP3K14 |
R-AG-Target gene: | Upregulation//-- |
Subcategory: | Unclear |
Target gene experiment: | qRT-PCR//Western blot |
Target gene description: | Using quantitative realtime PCR (qRT-PCR) in A375 melanoma cells, we observed a marked decrease in EZH2 mRNA under treatment with these NF-kB inhibitors.Similar to NF-kB2 silencing, NIK silencing strongly decreased the total NF-kB activity and abolished EZH2 promoter transcriptional activity. NF-kB2 overexpression (referred as o/e) in the melanocytes strongly increased EZH2 expression. |
Regulatory pathway: | -- |
R-AG-Pathway: | -- |
Pathway experiment: | -- |
Pathway description: | -- |
Annotation:
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