| Gene name: | FIS1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | Chang |
| Experiment: | SA-β-gal activity assay//Knockdown//Cell counting//XTT assay |
| Description: | The hFis1 knockdown cells started becoming progressively flattened on day 2 and were obviously flattened and enlarged on day 4, showing a phenotype similar to that of cells undergoing senescence. On days 4 and 5,~30% of the hFis1 RNAi cells stained positive for senescence-associated β-galactosidase , whereas no staining was observed in control cells. Microscopic evaluations revealed that the proliferation of hFis1-depleted cells was noticeably slow. Indeed, cell growth of the hFis1-depleted cells was found to be significantly retarded in measuring cell numbers and by XTT assays. |
| Target gene: | OPA1 |
| Official symbol(s): | OPA1 |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | SA-β-gal activity assay//Knockdown//Flow cytometry//Immunostaining |
| Target gene description: | Furthermore, hFis1 and OPA1 double-knockdown cells showed significant decreases in positive senescence-associated β-galactosidase staining as well as in cellular granularity.We found that prolonged depletion of hFis1 in fact increased ROS production on day 4. Moreover, sustained depletion of hFis1 caused a significant loss of mitochondrial membrane potential. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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