Aging Overview

Aging gene

Gene name: MAD2L1
Aging type: Prevent
Aging characteristic:
Tissue type: --
Cell name: IMR-90,MCF 10A
Gene ID: 4085
Category: protein coding
Phenotype: Aging
Experimental category: L
PMID: 22170163
Experiment: SA-β-gal activity assay//Immunostaining//SAHF
Description: Strikingly, several IMR90- siMAD2 cells were (SA)-β gal-positive (pH 6, 58%). The percentage of positive cells forgH2AX was higher (about 60%) in MCF10A-siMAD2 cells than in MCF10A-siGFP control cells .We then looked for SAHFs presence in primary human fibroblasts after 30 days from siMAD2 transfection and we scored that almost all of the cells (about 85%) showed heterochromatin foci when stained with DAPI.


Regulatory relationship

Target gene: P14
Official symbol(s): P14
R-AG-Target gene: --
Subcategory: Unclear
Target gene experiment: Western blot//qRT-PCR
Target gene description: At 72 h post-transfection, p14ARF mRNA was highly increased in IMR90 human fibroblasts. Similarly, Western-blotting experiments showed a high increase of p14ARF protein levels in IMR90- siMAD2 cells, suggesting that p14ARF could mediate the senescence response in these cells by sensing aneuploidy.

Regulatory pathway: P53-P21
R-AG-Pathway: --
Official symbol(s): TP53-CDKN1A
Pathway experiment: Western blot//qRT-PCR
Pathway description: Real-time RT-PCR revealed a high increase (sevenfold) of p21waf1 mRNA in primary human fibroblasts with MAD2 haploinsufficiency when compared to IMR90-siGFP control cells . As expected Western blotting confirmed that p21waf1 protein was significantly higher in siMAD2 than in IMR90-siGFP transfected cells. However, by Western blot analysis we detected elevated levels of p53 protein suggesting that the observed p21waf1 accumulation could be induced by p53 trans-activation of the p21waf1 gene. When p53 was posttranscriptionally silenced in IMR90-siMAD2 cells, expression level of the p21waf1 gene resulted similar to that present in IMR90-siGFP control cells, as assessed by real-time RT-PCR analysis.


Aging network

Annotation:

The green line represents Upregulation.

The purple line represents Downregulation.

The orange line represents Activation.

The yellow line represents Inhibition.

The gray line represents Unclear.



Pathway view

About risk SNP and eQTL

Loading,please wait...