| Gene name: | PTEN |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | MEF |
| Gene ID: | 5728 |
| Category: | protein coding |
| Phenotype: | Prostate cancer |
| Experimental category: | L |
| PMID: | 20197621 |
| Experiment: | SA-β-gal activity assay//Western blot |
| Description: | Pretreatment of Ptenlx/lx MEFs with aphidicolin,followed by acute inactivation of Pten,resulted in increased β-gal senescence staining and p53 accumulation after 24 hours. |
| Regulatory pathway: | PI3K-MTOR |
| R-AG-Pathway: | -- |
| Official symbol(s): | PIK3CA-MTOR |
| Pathway experiment: | SA-β-gal activity assay//Western blot |
| Pathway description: | Surprisingly,while?this?procedure?resulted?in?efficient?Pten?loss,?senescence?and?p53?levels?were?completely?abolished?by?rapamycin.?Wealso?tested?genetically?whether?mTOR?is?also?essential?for?PICS,? taking?advantage?of?Pten–/–mTOR–/–?double-null?MEFs,and?found this to?be?true. |
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