Aging Overview

Aging gene

Gene name: MYC
Aging type: Accelerate
Aging characteristic:
Tissue type: --
Cell name: IMR-90
Gene ID: 4609
Category: protein coding
Phenotype: Aging
Experimental category: HL
PMID: 29138277
Experiment: Crystal violet assay//SA-β-gal activity assay//BrdU assay//qRT-PCR//GSEA analysis
Description: Expression of the reprogramming factors (OSKM) in IMR90 human fibroblasts causes a senescence-like growth arrest that constitutes an intrinsic barrier to reprogramming (Banito et al. 2009). Similar to oncogenic RASG12V, the expression of OSKM induces the cyclin-dependent kinase (CDK) inhibitors (CDKIs) p15INK4b, p16INK4a, and p21CIP1, which are involved in implementing the stable growth arrest associated with senescence. Gene set enrichment analysis (GSEA) found signatures for senescence and the SASP significantly enriched in the transcriptome of cells expressing OSKM.


Regulatory relationship

Target gene: CDKN1A//MYOT//MTOR//UBE2E1
Official symbol(s): CDKN1A//MYOT//MTOR//UBE2E1
R-AG-Target gene: --//--//--//--
Subcategory: Unclear
Target gene experiment: Crystal violet staining//SA-β-gal activity assay//BrdU assay
Target gene description: The ability of shRNAs targeting CDKN1A, MYOT, MTOR, and UBE2E1 to prevent OSKM-induced senescence was confirmed by increased proliferation, a higher percentage of cells incorporating BrdU, and a decrease in the percentage of senescence-associated βgalactosidase (SA-β-Gal)-positive cells when compared with IMR90 cells infected with OSKM and a control vector .

Regulatory pathway: TGFβ
R-AG-Pathway: --
Official symbol(s): TGFB1
Pathway experiment: BrdU assay//Follow-up analysis
Pathway description: Moreover, inhibition of TGFBRI signaling blunted the growth arrest triggered by OSKM. In this regard, the scRNA-seq data and the follow-up analysis highlighted that TGF-β signaling was induced by OSKM .


Aging network

Annotation:

The green line represents Upregulation.

The purple line represents Downregulation.

The orange line represents Activation.

The yellow line represents Inhibition.

The gray line represents Unclear.



Pathway view

About risk SNP and eQTL

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