| Gene name: | CCND1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | MCF-7 |
| Gene ID: | 595 |
| Category: | protein coding |
| Phenotype: | Breast cancer |
| Experimental category: | L |
| PMID: | 29880532 |
| Experiment: | Cell morphological analysis//SA-β-gal activity assay//Immunostaining//SAHF//Knockdown |
| Description: | Cyclin D1-depleted cells appeared flattened with large volumes of cytoplasm and were positive for SA-β-Gal staining. Cyclin D1-depleted cells also showed a significant increase in senescence-associated heterochromatin foci (SAHF), another putative marker for cellular senescence (Narita et al., 2003). |
| Regulatory pathway: | P38-FOXO3A-P27 |
| R-AG-Pathway: | -- |
| Official symbol(s): | MAPK14-FOXO3-PSMD9 |
| Pathway experiment: | Western blot//Immunostaining//Cell counting |
| Pathway description: | We detected upregulation of phospho-p38 (Thr180/Tyr182) and JNK-mediated c-JUN phosphorylation at Ser73 in cyclin D1- depleted cells around 48-72 h after cyclin D1 depletion, but not in control cells.We detected an upregulation of FOXO3a protein in associatio nwith increased FOXO3a Ser7 phosphorylation, which is involved in the stress-induced activation of FOXO3a by p38 and translocalization of FOXO3a from the cytoplasm to the nucleus after cyclin D1 depletion. We found that p27 protein level was also upregulated in cyclin D1-depleted cells, whereas GADD45a and SOD2 were not upregulated at the protein level . |
Annotation:
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