| Gene name: | PAK4 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | Hs 578T |
| Gene ID: | 10298 |
| Category: | protein coding |
| Phenotype: | Breast cancer |
| Experimental category: | HL |
| PMID: | 31399573 |
| Experiment: | Cell morphological analysis//SA-β-gal activity assay//Western blot//BrdU assay |
| Description: | After transient transfection of two independent small interfering RNAs (siRNAs) targeting the human PAK4 gene, Hs 578T breast cancer cells adopted a flatter and larger senescenceassociated morphology and exhibited elevated SA-β-gal activity (as measured with the two substrates X-Gal29and MUG30) that was accompanied by a significant decrease in BrdU-incorporation.Genes involved in cell cycle arrest, DNA damage/ repair, and SASP factors are typically upregulated in senescent cells. PAK4 knockdown also increased protein expression of the known senescence-regulators p53 and p21. |
| Target gene: | RELB |
| Official symbol(s): | RELB |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Phosphorylation |
| Target gene experiment: | Western blot |
| Target gene description: | This inverse correlation was also observed at the protein level in Hs 578T breast cancer cells where PAK4 knockdown upregulated RELB. Considering expression as continuous vari- ables, the expression of PAK4 and RELB displayed the strongest significant inverse association . |
| Regulatory pathway: | NF-κB |
| R-AG-Pathway: | Downregulation |
| Official symbol(s): | NFKB1 |
| Pathway experiment: | qRT-PCR |
| Pathway description: | PAK4 inhibits NF-κB signaling.Upregulation of several NF-κB target genes upon PAK4 knockdown was validated by RT-qPCR in Hs 578T cells, including the NF-κB subunits NFKB1, NFKB2, and RELB as well as the previously characterized NF-κB response genes CD82, S100A4, TIMP2, CDKN1C, PRKCD, TWIST1, SPP1, TP53, and TRAF2. |
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