| Gene name: | NEF |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | MSC |
| Experiment: | SA-β-gal activity assay//Western blot |
| Description: | In control cells, the percentage of senescent cells X-gal stained was 8.6±2.2% and 9.3 ± 2.2%, after 10 and 20 days of culture, respectively. After 10 days, senescence was increased in cells treated with Tat+Nef, and after 20 days, its level was of 17.1 ± 1.6%, 18.0 ± 4.1% and 20.2 ± 3.7% in Tat-, Nef- and Tat+Nef-treated cells, respectively. |
| Target gene: | BECLIN1 |
| Official symbol(s): | BECLIN1 |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Immunostaining//Co-IP |
| Target gene description: | The cellular oxidase activity, was unchanged after 10 days of treatment with the HIV proteins, but increased after 20 days by 1.3- to 1.4-fold by Tat and/or Nef, when compared to control cells along with an increased superoxide dismutase activity (SOD).Moreover, we observed a direct interaction between Nefand Beclin-1, which were co-immunoprecipitated, suggesting that Nef could inhibit autophagy through a direct interaction with Beclin-1. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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