| Gene name: | NR2E1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | LNCaP,DU 145 |
| Gene ID: | 7101 |
| Category: | protein coding |
| Phenotype: | Prostate cancer |
| Experimental category: | L |
| PMID: | 25557355 |
| Experiment: | SA-β-gal activity assay |
| Description: | SA-β-Gal analysis revealed that there was a significant reduction of SA-β-Gal-positive cells in LNCaPTLX infectants compared to LNCaP-pBABE infectants.Cytochemical analysis on SA-β-Gal activity revealed that there was a dramatic increase of SA-β-Gal-positive cells in both LNCaP-shTLX and DU145-shTLX infectants as compared to Scramble-shRNA infectants. |
| Target gene: | P21//SIRT1 |
| Official symbol(s): | P21//SIRT1 |
| R-AG-Target gene: | Downregulation//Upregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot//CHIP |
| Target gene description: | Immunoblot analysis of senescence-associated markers showed that the expression level of cyclin-dependent kinase inhibitor p21WAF1/CIP1 (hereafter as p21) was markedly suppressed or undetected in LNCaP-/PC-3-TLX infectants but became upregulated in shTLX-infectants of both LNCaP and DU145 cells.ChIP analysis performed in prostate cancer cells identified that there was a significant enrichment of TLX at one consensus TLX-binding motif (TTCAGT) located at - 656 -650 bp and also a palindromic sequence (ACTGAA) at -616 -610 bp in the proximal CDKN1A (p21) promoter.Interestingly, we also detected a significant elevation of protein expression of an NAD- dependent protein deacetylase Sirtuin-1 (SIRT1) in LNCaP-TLX infectants but a reduction in shTLX-infectants by immunoblot analysis.ChIP assay showed that a proximal region of human SIRT1 gene promoter (-129 ? +32 bp) could be immunoprecipitated in TLX- and vector-transfected prostate cancer cells. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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