| Gene name: | CAV1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | WI-38,IMR-90 |
| Experiment: | SA-β-gal activity assay//Western blot//Knockdown |
| Description: | We found that down-regulation of caveolin-1 by shRNA was sufficient to induce cellular senescence in both WI-38 and IMR-90 cells, as quantified by senescence-associated β-galactosidase activity (SA-β-gal) staining and immunoblot analysis for p21, a marker of cellular senescence. |
| Target gene: | IFT88//AURKA |
| Official symbol(s): | IFT88//AURKA |
| R-AG-Target gene: | --//-- |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot//Immunostaining//SA-β-gal activity assay |
| Target gene description: | More specifically, because down-regulation of IFT88 is known to inhibit ciliogenesis, IFT88 protein expression was downregulated by siRNA in caveoli n-1–lacking WI-38 cells. We found that, when primary cilia formation was prevented by IFT88 down-regulation in caveolin-1–lacking cells, cellular senescence was dramatically inhibited .To this end, caveolin-1 deficiency was achieved by shRNA in both WI-38 and IMR-90 cells, and the protein levels of AURKA were quantified by immunoblotting analysis. AURKA was virtually lost in caveolin-1 lacking cells. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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