| Gene name: | SIRT1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | SH-SY5Y |
| Gene ID: | 23411 |
| Category: | protein coding |
| Phenotype: | Alzheimer's disease |
| Experimental category: | L |
| PMID: | 28962864 |
| Experiment: | MTT assay //CCK-8 assay |
| Description: | We further found that siSIRT1 treatment resulted in inhibited cell proliferation as indicated by the MTT assay and the CCK8 assay in a time course monitoring days 1 to 7. |
| Target gene: | P53//CREB |
| Official symbol(s): | P53//CREB1 |
| R-AG-Target gene: | --//Inhibition |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot |
| Target gene description: | We further found that siSIRT1 also inhibited CREB phosphorylation and enhanced p53 expression. |
| Regulatory pathway: | PI3K-AKT |
| R-AG-Pathway: | -- |
| Official symbol(s): | PIK3CA-AKT1 |
| Pathway experiment: | Western blot//Immunofluorescence |
| Pathway description: | Using the Western blotting assay, we observed that SIRT1 expression was absent in the siSIRT1-treated cells, but the PI3K levels were similar regardless of the treatment. AKT phosphorylation at the 308 site was inhibited by siSIRT1 treatment while the phosphorylation at the 473 site was similar among all of the three groups, indicating that SIRT1 regulates AKT phosphorylation, specifically at the 308 site.We next examined the subcellular localization of SIRT1 and AKT using immunostaining analysis, and the results showed that AKT was activated in these cells as indicated by plasma membrane localization. |
Annotation:
Loading,please wait...