| Gene name: | CD81 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | Bone,Muscle,Adipose,Hair,Lung |
| Cell name: | A549 |
| Gene ID: | 975 |
| Category: | protein coding |
| Phenotype: | Chronic obstructive pulmonary disease |
| Experimental category: | HL |
| PMID: | 29572511 |
| Experiment: | Histological staining//Cell morphological analysis//SA-β-gal activity assay//Flow cytometry |
| Description: | At 80 weeks of age, CD9/CD81 DKO mice were smaller and had less hair of a brownish color than WT mice, although DKO and WT mice could not be distinguished at 3 wk of age .Moreover, DKO mice developed progressive kyphosis and decreased bone mineral density. Muscle and visceral adipose tissue were significantly reduced in volume, as determined by CT quantitation.Consequently, DKO mice had remarkably shorter survival than WT mice. Histological examination revealed that DKO mice developed emphysema and osteoporosis at 80 wk. Moreover, Although the CD4/CD8 ratio, one of markers in immunosenesence, was not altered in younger mice, it was reduced in aged DKO mice in comparison with WT mice.Double knock-down (DKD) of CD9/CD81 in epithelial cells resulted in cells with a large flattened morphology, and the proportion of SA-β-Gal-positive cells increased. |
| Regulatory pathway: | SIRT1 |
| R-AG-Pathway: | -- |
| Official symbol(s): | SIRT1 |
| Pathway experiment: | Western blot//ELISA//Knockdown |
| Pathway description: | Notably, knockdown of CD9 and CD81 in epithelial cells additively downregulated the expression of SIRT1, whereas knockdown of CD151 did not. The reduced expression of SIRT1 was further verified by immunocytochemistry and ELISA. |
Annotation:
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