| Gene name: | CIZ1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | Brain |
| Cell name: | MEF |
| Gene ID: | 25792 |
| Category: | protein coding |
| Phenotype: | Neurodegenerative disease |
| Experimental category: | L |
| PMID: | 29154038 |
| Experiment: | Flow cytometry//Comet assay//Behavioral assessment//Immunostaining |
| Description: | Using flow cytometry, MEFs from null mice showed cell-cycle defects and increased apoptosis (two-tailed t(8) = 3.14, P = 0.014) in comparsion with MEFs from WT littermates. For this purpose, we investigated DNA damage in the cerebellum and hippocampus of aged WT and null mice with comet assays and immunohistochemistry. We found increased % tail DNA in the cerebellum (two-tailed t(10) = 12.14, P <0.0001) and hippocampus (two-tailed t(10) = 14.07,P <0.0001) of aged Ciz1?/ ? mice as compared with WT littermates .Behaviorally, male and female Ciz1?/ ? mice were more aggressive than their WT littermates in the dominance tube (P < 0.0001). Similar to motor and behavioral findings, we also observed cognitive dysfunction in Ciz1?/ ? mice as assessed by cross maze and Morris water maze testing. |
| Regulatory pathway: | NF-κB |
| R-AG-Pathway: | -- |
| Official symbol(s): | NFKB1 |
| Pathway experiment: | Western blot |
| Pathway description: | Consistent with our gene expression data, we observed increased protein levels of NF-κB (p65) in the nuclear extracts from the cerebellae and hippocampi. |
Annotation:
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