| Gene name: | DLX3 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | B-MSC |
| Experiment: | SA-β-gal activity assay//qRT-PCR//Western blot |
| Description: | The number of SA-β -gal+ staining cells 38% in TDO-BMSCs and 55% in CON-BMSCs suggested that BMSCs with DLX3 mutation remained a younger status while the normal BMSCs entered a premature senescence.After 72 h osteoinduction, aging-related markers p16INK4a and p15INK4b mRNA expression detected by real-time PCR and p16INK4a and GLB1 protein expression examined by western blot were significantly increased in WT-DLX3 but decreased in MT-DLX3 and TR-DLX3 when compared with EGFP-EV . Stemness markers Oct4 and Nanog mRNA expression detected by real-time PCR were significantly decreased in WT-DLX3 but increased in MT-DLX3 and TR-DLX3 when compared with EGFP-EV .The results showed that percentage of SA-β -gal-positive cells was significantly higher in WT-DLX3, but much lower in MT-DLX3 and TR-DLX3. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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