| Gene name: | ITGB3 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | BF |
| Experiment: | BrdU assay//SA-β-gal activity assay//Immunostaining//qRT-PCR |
| Description: | Indeed, expression of a retroviral vector encoding ITGB3 in BFs reduces their proliferation rate, quantified by measuring the percentage of cells incorporating bromodeoxyuridine (BrdU).Consistent with the activation of senescence, ITGB3 expression led to an increase in the number of cells staining positive for senescence-associated β-galactosidase (SA-β-Gal) activity, an accumulation of reActivate oxygen species (ROS), and a mild increase in the mRNA levels of different SASPs. |
| Target gene: | CBX7 |
| Official symbol(s): | CBX7 |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot//Immunostaining//Knockdown//CHIP |
| Target gene description: | Importantly,we observed that shCBX7 increases the number of cells presenting avβ3-stained FA complexes by IF. The regulation of β3 protein levels by CBX7 was also confirmed in IMR-90 fibroblasts.We checked the levels of the integrin heterodimer avb3 by immunofluorescence (IF)and the β3 subunit by immunoblot upon CBX7 knockdown or Cbx7 ectopic expression.Our data show a reduced binding of CBX7 to the ITGB3 TSS during OIS, suggesting that the endogenous upregulation of ITGB3 during OIS is due to the transcriptional deregulation of the locus by the loss of CBX7 binding. |
| Regulatory pathway: | P53-P21//TGF-β |
| R-AG-Pathway: | Activation//Activation |
| Official symbol(s): | TP53-CDKN1A// |
| Pathway experiment: | SA-β-gal activity assay//qRT-PCR |
| Pathway description: | Using a previously characterized shRNA targeting TP53 (shp53) , we impaired not only the proliferation arrest induced by ITGB3 but also the increase in SA-β-Gal activity. The use of a short interfering RNA (siRNA) targeting TP53 (sip53) also impaired the growth arrest induced by ITGB3 expression. Our data demonstrate that both siRNAs against TGFBR2 overcome senescence induced by the overexpression of ITGB3, as shown by measuring the relative cell number and p21CIPlevels by IF. Indeed, qPCR analyses of a range of regulators implicated in the TGF-β pathway are upregulated in BFs expressing ITGB3. |
Annotation:
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