| Gene name: | CXCR4 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | SH-SY5Y |
| Gene ID: | 7852 |
| Category: | protein coding |
| Phenotype: | Alzheimer's disease |
| Experimental category: | L |
| PMID: | 30080220 |
| Experiment: | CCK-8 assay//MTT assay//Knockdown |
| Description: | Moreover, the data of CCK8 assay demonstrated that the cell activity was decreased in siCXCR4 cells. Meanwhile,siCXCR4 cells have significant deficient effects on cell proliferation and activity compared with normal and control groups. |
| Target gene: | AKT//CREB//P53 |
| Official symbol(s): | AKT//CREB1//P53 |
| R-AG-Target gene: | Activation//Activation//Activation |
| Subcategory: | Phosphorylation |
| Target gene experiment: | Western blot//Immunostaining//Knockdown |
| Target gene description: | Using Western Blotting assay, we observed that the phosphorylation at 308 of AKT in siCXCR4 group was robustly inhibited.Using a pixelby-pixel colocalization analysis, the results illustrated that fluorescent signals of AKT were clearly merged with CXCR4 on plasma membrane. Meanwhile, the phosphorylation of CREB was significantly decreased, while the level of P53 was increased in CXCR4 knock down cells compared with normal or control groups. |
| Regulatory pathway: | CXCL12-CXCR4 |
| R-AG-Pathway: | Activation |
| Official symbol(s): | CXCL12-CXCR4 |
| Pathway experiment: | Immunostaining |
| Pathway description: | We firstly found a strong colocalization of CXCR4 and AKT on plasma membrane stimulating by 100ng/ml CXCL12. |
Annotation:
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