| Gene name: | PLA2R1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | MRC-5 |
| Gene ID: | 22925 |
| Category: | protein coding |
| Phenotype: | Hutchinson–Gilford progeria syndrome |
| Experimental category: | L |
| PMID: | 30216637 |
| Experiment: | qRT-PCR//SA-β-gal activity assay//Cell counting//Crystal violet assay//Knockdown |
| Description: | Constitutive expression of progerin resulted in proliferation arrest as judged by reduced number of cells observed using crystal violet staining and growth curves and reduced expression of the proliferation marker Ki67, and increased frequency of SA‐β‐Gal‐positive cells and increased expression of p21 (CDKN1A) and the SASP component IL‐8.Knockdown of PLA2R1 with two independent shRNAs abolished all these hallmarks of cellular senescence. |
| Regulatory pathway: | P53-FDPS |
| R-AG-Pathway: | -- |
| Official symbol(s): | TP53-FDPS |
| Pathway experiment: | qRT-PCR//Immunostaining |
| Pathway description: | As expected,progerin increased P‐ATM and γH2AX DNA damage marks, p53 phosphorylation, the p53 transcriptional target p21, and FDPS, and these inductions were abolished upon PLA2R1 knockdown. |
Annotation:
Loading,please wait...