| Gene name: | SIRT1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | PAEC |
| Experiment: | SA-β-gal activity assay//Flow cytometry |
| Description: | Results from SA-β-gal staining, as well as flow cytometric analysis, suggested that SIRT1 alleviated cellular senescence. |
| Regulatory pathway: | LKB1-AMPK//AKT |
| R-AG-Pathway: | Activation//Activation |
| Official symbol(s): | STK11-PRKAA1//AKT1 |
| Pathway experiment: | Western blot//SA-β-gal activity assay |
| Pathway description: | Importantly, the amount of LKB1 and phosphoAMPK(T172) were significantly lower in SIRT1 transgenic mice. In addition, decreased SIRT1 expression and elevated LKB1/AMPK levels were also observed in the aorta tissues of old mice by comparing to those in young mice.SIRT1 and resveratrol treatment increased Akt(Ser473) phosphorylation in normal cultures,Inhibition of Akt by either Akt inhibitor or kinase-dead Akt could induce senescence only when the experiment was performed under serum containing conditions .These results largely mirrored the effects of Akt inhibition on AMPK activation. |
Annotation:
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