| Gene name: | WRN |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | WI-38 |
| Gene ID: | 7486 |
| Category: | protein coding |
| Phenotype: | Werner syndrome |
| Experimental category: | HL |
| PMID: | 16287861 |
| Experiment: | Cell morphological analysis//SA-β-gal activity assay//Flow cytometry//BrdU assay |
| Description: | We found that upon withdrawal of WRN, the morphology of untransformed primary fibroblasts changed progressively within 5 days posttransfection. The cells became enlarged, flattened and developed the SA-β-Gal activity with a concomitant moderate decline of cumulative cell number.The significant hypophosphorylation of Rb was accompanied by decreased proliferation of the WRN-deficient cells, as evidenced by an average 40% reduction of bromodeoxyuridine incorporation (data not shown) and a similar decrease in S-phase cells with an increased proportion of G0-G1 cells by FACS analysis. |
| Target gene: | P16 |
| Official symbol(s): | P16 |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | Knockdown//Western blot |
| Target gene description: | Untransformed fibroblasts responded to acute knockdown of WRN with a minimal elevation of the levels of the p16 cell-cycle inhibitors within the time frame of 5 days. |
| Regulatory pathway: | P53//RB |
| R-AG-Pathway: | --//-- |
| Official symbol(s): | TP53//retinoblastoma |
| Pathway experiment: | Knockdown//Western blot |
| Pathway description: | Untransformed fibroblasts responded to acute knockdown of WRN with a minimal elevation of the levels of p21,the downstream p53 target within the time frame of 5 days. However,an almost complete disappearance of retinoblastoma (Rb) phosphorylation and, to a lesser degree, the dephosphorylated Rb protein was evident . |
Annotation:
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