| Gene name: | PNPT1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HeLa |
| Experiment: | Flow cytometry//Western blot |
| Description: | Overexpression of hPNPaseold-35 induces a senescence-like growth arrest and also generates ROS. |
| Regulatory pathway: | NF-κB |
| R-AG-Pathway: | Activation |
| Official symbol(s): | NFKB1 |
| Pathway experiment: | Western blot//RT-PCR//ELISA//Luciferase reporter assay |
| Pathway description: | However,infection with Ad.hPNPaseold -35 resulted in a 10- to 12-fold induction in relative luciferase activity in comparison with control or Ad.vec-infected cells.On Ad.hPNPaseold-35 infection, the binding pattern changed, with the p50/p50 homodimer disappearing, and the binding of the p50/p65 heterodimer increasing markedly.Expressions of mRNAs and secreted proteins of IL-6 and IL-8, two NF-kB target genes, were analyzed by RT-PCR and ELISA, respectively,after Ad.hPNPaseold-35infection. |
Annotation:
Loading,please wait...