| Gene name: | AKT1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | BJ-T |
| Experiment: | SA-β-gal activity assay//Cell morphological analysis//qRT-PCR |
| Description: | Cells expressing activated AKT showed a significant increase in SAβGAL and cell size.AKT-induced senescence is characterised by an SASP, with increased secretion of IL-1a, IL-1b, IL-6 and IL-8. |
| Regulatory pathway: | P53//MTORC1 |
| R-AG-Pathway: | Upregulation//-- |
| Official symbol(s): | TP53//NULL |
| Pathway experiment: | Knockdown//SA-β-gal activity assay//Western blot |
| Pathway description: | SAβGAL positivity was significantly reduced in BJ-T-myr-AKT/p53 stable knockdown cells as compared with control BJ-T-myr-AKT cells. Acute p53 knockdown, confirmed by immunoblot analysis, rescued the reduced proliferation of myr-AKT1-expressing cells to that of control cells .Upon treatment with rapamycin,the percentage of AKT cells positive for SAβGAL was significantly reduced .Rapamycin treatment also dramatically reduced AKT-induced effects on cell size, and the SASP, indicating that mTORC1 activity is critical for PI3K/AKT-driven senescence. |
Annotation:
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