| Gene name: | SPAG9 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | MDA-MB-231 |
| Gene ID: | 9043 |
| Category: | protein coding |
| Phenotype: | Breast cancer |
| Experimental category: | L |
| PMID: | 27449044 |
| Experiment: | Cell cycle analysis//Knockdown//Western blot//SA-β-gal activity assay |
| Description: | Knockdown of SPAG9 by SPAG9 shRNA1(67.03 %) and shRNA2 (65.14 %) resulted in accumulation of most of the cells in G1 phase as compared to NC shRNA (63.50 %) transfected cells. Also, the percentage of G2-M phase cells showed decrease in SPAG9 shRNA [shRNA1 (23.08 %), shRNA2 (26.42 %)]-transfected cells as compared to NC shRNA (28.26 %)-transfected cells.The result showed that there was a significant decrease in cyclins and cyclin-dependent kinases such as cyclin B1, cyclin D1, cyclin E,CDK1, CDK4, and CDK6. Also, upregulation was observed in case of tumor suppressor protein, p21. The percentage of senescent cells was significantly higher(p < 0.0001), 52.6 % and 67.0 %, when transfected with SPAG9 shRNA1 and shRNA2, respectively, as compared to 7.4 % when transfected with NC shRNA. |
| Target gene: | P21//CYCLIN B1//CYCLIN D1//CYCLIN E//CDK4//CDK6 |
| Official symbol(s): | P21//CYCLIN B1//CYCLIN D1//CYCLIN E//CDK4//CDK6 |
| R-AG-Target gene: | --//--//--//--//--//-- |
| Subcategory: | Unclear |
| Target gene experiment: | Immunostaining//Western blot |
| Target gene description: | IHC analysis of tumor serial sections revealed significantly enhanced immunoreactivity of p21 and decreased immunoreactivity of cyclin B1, cyclin D1,cyclin E, CDK4, and CDK6 in SPAG9 shRNA2-treated mice as compared NC shRNA-treated mice.Western blotting was carried out to study the various molecules in different phases of cell cycle, which showed that there was a significant decrease in cyclins and cyclindependent kinases such as cyclin B1, cyclin D1, cyclin E,CDK1, CDK4, and CDK6. Also, upregulation was observed in case of tumor suppressor protein, p21. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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