| Gene name: | TNF |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | ECFC |
| Experiment: | SA-β-gal activity assay |
| Description: | We incubated ECFCs with either recombinant TACE or an anti-TNFR2 neutralizing antibody for 6 days and determined development of premature senescence by staining for senescence-associated β-galactosidase. |
| Target gene: | P38 |
| Official symbol(s): | P38 |
| R-AG-Target gene: | Activation |
| Subcategory: | Unclear |
| Target gene experiment: | SA-β-gal activity assay//Colony formation assay |
| Target gene description: | We examined the effect of p38 inhibition on the expression of tmTNF and found that blocking p38 completely prevented the loss of tmTNF and subsequent development of premature senescence. |
| Regulatory pathway: | TMTNF-TNFR2 |
| R-AG-Pathway: | -- |
| Official symbol(s): | TNF-TNFRSF1B |
| Pathway experiment: | Western blot |
| Pathway description: | We treated ECFCs with either TACE or anti-TNFR2 neutralizing antibody and detected the presence of p16ink, a senescence-associated cell cycle regulating protein by Western blot and found that p16ink levels increased dramatically during the course of the 6-day treatment, further confirming that loss of the tmTNF/TNFR2 signaling axis results in premature senescence of ECFCs. |
Annotation:
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