| Gene name: | CXCL8 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | PMSC |
| Experiment: | Cell morphological analysis//SA-β-gal activity assay//Flow cytometry//CCK-8 assay |
| Description: | The enlarged and flattened PMSCs stained blue, indicating they have become senescent. The percentage of SA-β-gal-positive PMSCs was significantly increased on day9 after IL-8 knockdown.A flow cytometric analysis indicated that the cellular G2/M phase ratio was significantly increased, indicating that the G2-M phase was prolonged in the IL-8-silenced PMSCs when compared with their controls.The IL-8-silenced PMSCs showed significant growth retardation. |
| Regulatory pathway: | AKT-FOXO3A//CXCR2 |
| R-AG-Pathway: | --//-- |
| Official symbol(s): | AKT1-FOXO3//CXCR2 |
| Pathway experiment: | qRT-PCR//Western blot |
| Pathway description: | Increased p-AKT expression and decreased FOXO3a protein expression in IL-8-silenced PMSCs were verified by western blot analysis.We found that in IL-8-silenced PMSCs, the expression levels of CXCR2 ligands were time-dependent: most of the CXCR2 ligands were present at reduced levels in pre-senescent shIL-8 PMSCs,while their expression later increased along with the cellular senescence process. |
Annotation:
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