| Gene name: | DUSP4 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | WI-38 |
| Experiment: | Cell morphological analysis//SA-β-gal activity assay//MTT assay |
| Description: | In contrast to the vigorously proliferating, spindle-like control fibroblasts, MKP2 cells exhibit the distinct flattened, enlarged morphology of senescent cells, sensitivity to contact inhibition, and loss of replicative capacity.we found that throughout their lifespan, the percentage of stained cells was always higher in the cultures expressing MKP2. |
| Regulatory pathway: | ERK |
| R-AG-Pathway: | Downregulation |
| Official symbol(s): | MAPK1 |
| Pathway experiment: | Knockdown |
| Pathway description: | MKP2 expression resulted in a dramatic decrease of thelifespan of the ERK2:NLS line while it had no effect in the lifespan of the phosphatase resistant ERK2(D319N):NLS cells. Thus, we concluded that MKP2 overexpression inhibits proliferation and accelerates senescence by inhibiting nuclear ERK signaling and not by inactivating other MAPK family members. |
Annotation:
Loading,please wait...