| Gene name: | CCN1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | A549,NCI-H460,NCI-H520 |
| Gene ID: | 3491 |
| Category: | protein coding |
| Phenotype: | Lung cancer |
| Experimental category: | L |
| PMID: | 23553737 |
| Experiment: | Flow cytometry//Cell morphological analysis//SA-β-gal activity assay//BrdU assay |
| Description: | The CCN1-treated H460 cells showed prominent growth retardation starting at passage number 8. For A549 and H520 cells,significant growth suppression was also observed starting at passage number 11 and 13,respectively；Analysis by flow cytometry for cell cycle distributions of H460, A549, and H520 cells at passage number 11, 14, and 16, respectively, indicated that CCN1 induced cell cycle arrest in the G1 phase in all three NSCLC cell lines. The CCN1-treated cells exhibited a flattened morphology with concomitant cell enlargement, the presence of vacuole-rich cytoplasm, and high expression of perinuclear senescence-associated-β-galactosidase.Addition of CCN1 reduced BrdU incorporation to ~20 % of that observed in control samples with no or mock treatment in all three cell lines, suggesting the cessation of DNA synthesis in the senescence-like cells. |
| Regulatory pathway: | P53-P21 |
| R-AG-Pathway: | Upregulation |
| Official symbol(s): | TP53-CDKN1A |
| Pathway experiment: | Cell morphological analysis//SA-β-gal activity assay//Knockdown |
| Pathway description: | Addition of CCN1 to the p21-deficient cells failed to suppress cell growth, to induce senescence-like morphologies (6C), or to enhance SA-β-Gal activities (6D). Similar results were found in the p53-knockdown cells. |
Annotation:
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