| Gene name: | CXCR2 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | Embryo |
| Cell name: | MEF,IMR-90 |
| Experiment: | SA-β-gal activity assay//BrdU assay//Immunostaining//Growth curve assay |
| Description: | When viruses expressing CXCR2 were used to infect IMR-90 cells,both caused retarded growth culminating in premature senescence.The proportion of cells staining positively for senescence-associated β-ga-lactosidase (β-Gal) activity and displaying senescence-associated heterochromatin foci was elevated in cultures transduced with CXCR1 compared with controls.The cells showed reduced incorporation of BrdU and displayed characteristic features of senescence. |
| Regulatory pathway: | P53//RB |
| R-AG-Pathway: | --//-- |
| Official symbol(s): | TP53//retinoblastoma |
| Pathway experiment: | Crystal violet staining |
| Pathway description: | To this end we analyzed the effects of CXCR2 in WI-38 human fibroblasts in which p53 or pRb functions were disrupted using the HPV E6 and E7 proteins, respectively. The data confirm the importance of p53 but imply that the Rb pathway is also involved in establishing the CXCR2-induced arrest in human cells. |
Annotation:
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