| Gene name: | E6 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | SiHa |
| Gene ID: | 1489078 |
| Category: | protein coding |
| Phenotype: | Cervical cancer |
| Experimental category: | L |
| PMID: | 17586029 |
| Experiment: | Cell morphological analysis//SA-β-gal activity assay//Knockdown |
| Description: | The depression of E6 caused the SiHa/16AS cells to cease proliferation and adopt a flattened and enlarged appearance, suggesting that the cells efficiently underwent senescence.The colonies arising from the mock-transfected showed faint background staining, whereas almost all of the cells transfected with 16AS displayed intense blue staining indicative of SA-β-gal activity and senescence. |
| Regulatory pathway: | P53-RB |
| R-AG-Pathway: | -- |
| Official symbol(s): | RB1-TP53 |
| Pathway experiment: | Western blot |
| Pathway description: | Downregulation of HPV-16 E6 and E7 by 16AS transfection resulted in remarkably elevation of p53 expression in SiHa cells. Furthermore, the introduction of 16AS led to an obvious increase in the levels of hypophosphorylated p105Rb. |
Annotation:
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