| Gene name: | HDAC6 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | Periodontal Ligament |
| Cell name: | PDLSC |
| Experiment: | MTT assay//Colony formation assay//SA-β-gal activity assay//Cell morphological analysis//BrdU assay |
| Description: | The results indicated that PDLSCs with HDAC6-inhibited by Rocilinostat or Tubastatin A displayed significantly lower proliferation rate compared with cells treated with DMSO as a control.Such inhibitory effect was further demonstrated using colony formation assay, as limited crystal violet staining of stable clones observed in HDAC6-inhibited cells versus control cells;Characteristic morphologies of senescence, including elevated SA-β-gal activity, enlarged and flattened cell size, and accumulation of granular cytoplasmic inclusions could be observed in Rocilinostat or Tubastatin A treated cells rather than control cells;Moreover, reduced BrdU incorporation further supported the pro-senescence action of Rocilinostat and Tubastatin A . |
| Target gene: | P27 |
| Official symbol(s): | P27 |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Acetylation |
| Target gene experiment: | Western blot |
| Target gene description: | We overexpressed HDAC6 and found significantly decreased p27Kip1 acetylation upon HDAC6 overexpression.Moreover, ectopic expression of HDAC6 also resulted in down-regulation of p27Kip1 proteins. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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