Gene name: | LMNA |
Aging type: | Accelerate |
Aging characteristic: |
Tissue type: | Umbilical cord blood |
Cell name: | MSC |
Experiment: | Western blot//SA-β-gal activity assay//MTT assay |
Description: | Immunoblots confirmed the over-expression of GFP-progerin and increased expression of p16INK4a, a marker of senescence in hMSCs;Induction of cellular senescence was also confirmed by the increase in SA-β-gal activity;MTT activity was decreased in response to progerin over-expression, indicating decreased proliferative activity. |
Target gene: | MCP-1//NF-?B |
Official symbol(s): | CCL2//NFKB1 |
R-AG-Target gene: | Upregulation//Upregulation |
Subcategory: | Unclear |
Target gene experiment: | qRT-PCR//ELISA//Western blot//Immunostaining |
Target gene description: | Except for MCP-1, most cytokines that have been reported to increase in senescent fibroblasts were not increased in senescent hMSCs expressing progerin.The secretion of MCP-1 was also increased in the CM from progerin-expressing cells.The activation of NF-?B was also increased in response to progerin expression. |
Regulatory pathway: | -- |
R-AG-Pathway: | -- |
Pathway experiment: | -- |
Pathway description: | -- |
Annotation:
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